【Title】

Myocardial apoptosis and related gene expression in rats with acute myocardial infarction following bone marrow mononuclear cells transplantation

【Source】

Journal of Clinical Rehabilitative Tissue Engineering Research  October 1, 2009  Vol.13 No. 40，7898-7902

【Author】

Zhang Rui-cheng¹, Dong Nian-guo², Liang Kai¹, Hou Jian-feng³, Wu Gang³, Huang Xiao-yu³

【Setting】

¹Department of Cardiovascular Surgery, Henan Provincial Chest Hospital, Zhengzhou  450008, Henan Province, China; ²Department of Cardiovascular Surgery, Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan  430022, Hubei Province, China; ³Department of Cardiovascular Surgery, Second Hospital Affiliated to Zhengzhou University, Zhengzhou  450014, Henan Province, China

【Found】

【Abstract】

BACKGROUND: The increased cell apoptosis results in cadiocyte loss, which is the main reason for congestive heart failure after acute myocardial infarction (AMI). Myocardial apoptosis can be inhabited by decreasing tumor necrosis factor α (TNF-α) concentration, as well as the Bax protein, and PDCD5 mRNA expression.

OBJECTIVE: To discuss the influence of allogenic bone marrow mononuclear cells (BM-MNCs) transplantation to the myocardial apoptosis of rats and related gene expression after AMI.

DESIGN, TIME AND SETTING: The randomized control animal experiment was performed at the Affiliated Union Hospital Huazhong University of Science and Technology from January to November 2006.

MATERIALS: Sixty Wistar rats, with body weight of (240±20) g, were randomly divided into sham surgery, model and transplantation groups, with 20 animals in each group. The other 60 Wistar rats weighted 100-150 g were prepared for BM-MNCs.

METHODS: Rats in the model and transplantation groups were prepared for AMI models by ligating left anterior descending coronary artery. In the sham surgery group, rats’ chests were opened without ligating left anterior descending coronary artery. After model preparation, BM-MNCs suspension was implanted into infracted cardiac muscle via 4 points of epicardium with 100 μL per point in the transplantation group. The same volume DMEM was injected into rats of the sham surgery and model group. All rats were kept for 4 weeks.

MAIN OUTCOME MEASURES: The index of myocardial apoptosis, the TNF-α content in blood serum and in the myocardium of the left ventricular non-infarcted area, the Bax albumin content and the PDCD5mRNA expression of left ventricular non-infarcted area were measured. 

RESULTS: The BrdU labeled positive BM-MNCs distributed focally in the myocardial infarction area of the transplantation group at 4 weeks after transplantation. Compared to the sham surgery group, the TNF-α content in blood serum and in the left ventricular non-infarcted myocardium in the control and transplantation groups were increased notably at 4 weeks after transplantation (P < 0.05). However, the increased TNF-α content was less in the transplantation group than that of the model group (P < 0.05). The myocardial apoptosis index, Bax albumin and PDCD5mRNA of control group and transplantation group were all higher than that of sham surgery group (P < 0.05), but the increased amplitude was smaller in the transplantation group (P < 0.05).

CONCLUSION: Allogenic BM-MNCs transplantation can restrain myocardial apoptosis after AMI, which may be related to its effect on reduce TNF-α expression, decrease PDCD5 genetic promotion effect to apoptosis by restraining the expression of TNF-α and Bax protein.

【Number of pictures】

7 figures

【Full text】

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