Regulation of Wnt signaling pathway involved in the differentiation of hair follicle stem cells induced by lithium chloride

Regulation of Wnt signaling pathway involved in the differentiation of hair follicle stem cells induced by lithium chloride

Publisher：tangxyzxb Publish Time：Saturday, February 04, 2012
Source：crter

Abstract
BACKGROUND: Previous studies showed that hair follicle stem cells (HFSCs) can undergo committed differentiation induced by lithium chloride (LiCl), but relating molecular regulation mechanism undisclosed.
OBJECTIVE: To observe the changes of downstream molecules and target genes in Wnt signaling pathway during exogenous factor LiCl intervenes HFSCs and to investigate the role of LiCl in committed differentiation of HFSCs.
METHODS: HFSCs were isolated from incised scalps of patients treated with rhytidectomy by two-steps enzyme digestion followed surgical microscope operation. HFSCs were subjected to antibodies-fluorescein isothiocy of cytokeratin 19 for identification. HFSCs was intervened by exogenous factor LiCl.
RESULTS AND CONCLUSION: After 5 days HFSCs treated with LiCl, HFSCs volume got larger and the nucleus/cytoplasm ratio was reduced. In Wnt signaling pathway, the mRNA expression level of β-catenin and cyclinD1 increased, GSK3β and Tcf3 decreased, accompanied with c-myc increased at a lower concentration of LiCl and inversing decreased. LiCl can affect the expression level of β-catenin, GSK3β, Tcf3 ,c-myc and cyclinD1 involved in Wnt signal pathway, which may play an important role in HFSC differentiation.
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